NAD+ Pen Dosage Protocol

Quick Start

The short version

If you only read one thing, read this. The four cards below are the whole protocol in plain English — the sections that follow just add the detail.

Dosage Chart

Handling

Dosing & Reconstitution Guide

The pen is pre-filled — no reconstitution required. Prime the pen, dial the target mg dose, and inject slowly subcutaneously. Rapid delivery may cause a flushing sensation.

Subcutaneous Protocol

Frequency: 2–3× weekly subcutaneously. Inject slowly — rapid NAD+ delivery is associated with transient chest pressure and flushing that resolve when the rate is reduced.

Supplies Needed

Plain-English Glossary

Jargon, translated

A quick decoder for the terms you'll see across this page.

Subcutaneous (subQ)

Injected into the fatty layer just under the skin — not into muscle or vein. Easier and less painful than an IM shot.

Reconstitution

Mixing a dry powder with liquid (bacteriostatic water) to turn it into an injectable solution.

Lyophilized

Freeze-dried. The peptide arrives as a dry powder so it stays stable during shipping.

Bacteriostatic water

Sterile water with a tiny amount of preservative that prevents bacteria growth — used to mix peptides.

Units (on an insulin syringe)

The small tick marks on a U-100 syringe. 100 units = 1 mL. Most doses are just a few units.

Half-life

How long it takes your body to clear half of a dose. Guides how often you inject.

Titration

Starting low and slowly increasing the dose so your body can adjust.

Protocol Overview

• Goal: Support research on NAD+ metabolism, mitochondrial function, and longevity.
• Schedule: 2–3× weekly subcutaneous injection.
• Dose range: 50–300 mg per administration.
• Storage: Refrigerated, protected from light.

Dosing Protocol

• Start: 50 mg twice weekly.
• Titrate to 100–200 mg based on tolerability.
• Timing: Morning administration is common.
• Cycle length: 4–12 weeks in reference protocols.

Storage Instructions

• Lyophilized product: room temperature, sealed and protected from light. Refrigeration acceptable.
• Reconstituted product: 2–8 °C (35.6–46.4 °F) refrigerated, protected from light.
• Do not freeze reconstituted solution.
• Allow to reach room temperature before administration to reduce injection-site response.

Important Notes

• Use a new sterile syringe for each administration.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
• Discard the vial if the solution becomes cloudy, discolored, or shows particulates.
• Document daily dose and site rotation for consistency.
• Slow injection reduces flushing and chest-pressure sensations.

Mechanism

How This Works

Potential Benefits & Side Effects

• Reported improvements in energy and cognitive metrics in longevity research.
• Support for mitochondrial function in preclinical models.
• Increased sirtuin activity supporting metabolic regulation.
• Common transient adverse effects: flushing, chest tightness, nausea — rate-dependent.

Lifestyle Factors

• Combine with resistance training to leverage mitochondrial adaptation.
• Ensure adequate B-vitamin intake supporting NAD+ synthesis pathways.
• Prioritize sleep — NAD+ oscillates with circadian rhythm.
• Limit alcohol which depletes NAD+.

Injection Technique

1. 1. Attach a fresh pen needle and prime.
2. 2. Clean the injection site with alcohol.
3. 3. Dial the target mg dose.
4. 4. Pinch a skinfold; insert at 45–90° subcutaneously.
5. 5. Depress the plunger slowly to reduce flushing.
6. 6. Withdraw and dispose of the pen needle in a sharps container.

References

1. 1

   Yoshino et al., Cell Metab (2018)

   NAD+ intermediates and metabolic health

   View source
2. 2

   Rajman et al., Cell Metab (2018)

   Therapeutic potential of NAD-boosting molecules

   View source