5-Amino-1MQ Pen Dosage Protocol

Quick Start

The short version

If you only read one thing, read this. The four cards below are the whole protocol in plain English — the sections that follow just add the detail.

Dosage Chart

Handling

Dosing & Reconstitution Guide

The pen is pre-filled — no reconstitution required. Prime the pen, dial the target mg dose, and inject subcutaneously. Multiple pens are typically needed to reach the daily research dose.

Subcutaneous Protocol

Frequency: once daily subcutaneously. Morning administration is common in reference protocols.

Supplies Needed

Plain-English Glossary

Jargon, translated

A quick decoder for the terms you'll see across this page.

Subcutaneous (subQ)

Injected into the fatty layer just under the skin — not into muscle or vein. Easier and less painful than an IM shot.

Reconstitution

Mixing a dry powder with liquid (bacteriostatic water) to turn it into an injectable solution.

Lyophilized

Freeze-dried. The peptide arrives as a dry powder so it stays stable during shipping.

Bacteriostatic water

Sterile water with a tiny amount of preservative that prevents bacteria growth — used to mix peptides.

Units (on an insulin syringe)

The small tick marks on a U-100 syringe. 100 units = 1 mL. Most doses are just a few units.

Half-life

How long it takes your body to clear half of a dose. Guides how often you inject.

Titration

Starting low and slowly increasing the dose so your body can adjust.

Protocol Overview

• Goal: Support research on NNMT inhibition and NAD+ metabolism.
• Schedule: Daily subcutaneous injection.
• Dose range: 50–150 mg per administration.
• Storage: Refrigerated, protected from light.

Dosing Protocol

• Start: 50 mg once daily for one week.
• Target: 100 mg once daily as a standard reference dose.
• Timing: Morning administration is common.
• Cycle length: 8–12 weeks in reference protocols.

Storage Instructions

• Lyophilized product: room temperature, sealed and protected from light. Refrigeration acceptable.
• Reconstituted product: 2–8 °C (35.6–46.4 °F) refrigerated, protected from light.
• Do not freeze reconstituted solution.
• Allow to reach room temperature before administration to reduce injection-site response.

Important Notes

• Use a new sterile syringe for each administration.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
• Discard the vial if the solution becomes cloudy, discolored, or shows particulates.
• Document daily dose and site rotation for consistency.

Mechanism

How This Works

Potential Benefits & Side Effects

• Reduced fat mass and improved metabolic markers in preclinical models.
• Increased NAD+ and SIRT1 activity in adipose tissue research.
• Preservation of lean mass reported in animal studies.
• Investigational; human clinical data limited.

Lifestyle Factors

• Prioritize sleep — repair and regeneration are heavily circadian.
• Maintain adequate protein intake to support baseline anabolic processes.
• Stay well hydrated across the dosing cycle.
• Avoid alcohol on injection days to minimize additive vasodilation.

Injection Technique

1. 1. Attach a fresh pen needle and prime.
2. 2. Clean the injection site with alcohol.
3. 3. Dial the target mg dose.
4. 4. Pinch a skinfold; insert at 45–90° subcutaneously.
5. 5. Depress fully, hold 5–10 seconds, withdraw.
6. 6. Dispose of the needle in a sharps container.

References

1. 1

   Kannt et al., Sci Rep (2018)

   Small-molecule NNMT inhibitors reduce adiposity in diet-induced obesity

   View source
2. 2

   Neelakantan et al. (2018)

   Structural basis of 5-Amino-1MQ NNMT inhibition

   View source